| R64 | Grace Lorenz (WLU) def. Summer Williams (HAV) 6-2, 6-0 |
| Lauren Long (WLU) def. Amanda Hagino (UMW) 7-5, 6-2 |
| Raine Weis (CNU) def. Margaret Carlton (WLU) 6-7 (7-9), 6-2, 10-8 |
| Bin Zheng (CMU) def. Gabi Moss (WLU) 6-3, 6-4 |
| Izzy Koziol (WLU) def. Mackenzie Sherman (FMC) 6-1, 6-2 |
| Riho Iijima (WLU) def. Niamh Harding (JHU) 6-3, 3-6, 12-10 |
| Becket Waters (WLU) def. Tessa Trate (CNU) 6-4, 6-3 |
| Marta Mikos (WLU) def. Jenna Harrison (FMC) 6-4, 6-4 |
| R32 | Sarah Yang (CMU) def. Grace Lorenz (WLU) 6-1, 6-3 |
| Lauren Long (WLU) def. Malikaa Marathe (CMU) 6-4, 6-3 |
| Izzy Koziol (WLU) def. Martine Fierro (DC) 6-1, 6-1 |
| Jenny Lewis (SWA) def. Riho Iijima (WLU) 6-0, 6-2 |
| Becket Waters (WLU) def. Tessa Trate (CNU) 6-3, 6-1 |
| Marta Mikos (WLU) def. Danna Taylor (CMU) 6-4, 6-2 |
| R16 | Ruth de Souza (SBC) def. Lauren Long (WLU) 6-3, 0-6, 6-4 |
| Izzy Koziol (WLU) def. Divya Venkatarama (CMU) 6-3, 6-3 |
| Kamila Wong (JHU) def. Becket Waters (WLU) 6-3, 6-2 |
| Marta Mikos (WLU) def. Abby Moghtader (UMW) 7-5, 6-1 |
| QF | Crystal Zhou (CMU) def. Izzy Koziol (WLU) 6-1, 6-4 |
| Marta Mikos (WLU) def. Kamila Wong (JHU) 2-6, 6-4, 6-2 |
| SF | Crystal Zhou (CMU) def. Marta Mikos (WLU) 6-3, 2-6, 6-2 |
| C32 | Margaret Carlton (WLU) def. Hana Kimmey (UMW) 8-6 |
| Gabi Moss (WLU) def. Agatha Malinowski (SIT) 8-3 |
| C16 | Margaret Carlton (WLU) def. Cassidy Landau (FMC) 8-3 |
| Gabi Moss (WLU) def. Emily Beckner (UMW) 8-1 |
| CSF | Gabi Moss (WLU) def. Amanda Hagino (UMW) 8-6 |
| CFI | Gabi Moss (WLU) def. Zoe Angell (CMU) 8-4 |
| BAQF | Kaitlyn Chalker (WLU) def. Nikhita Simhambhatla (SBC) 8-4 |
| BASF | Kaitlyn Chalker (WLU) def. Stephanie Untermeyer (SIT) 8-2 |
| BAFI | Kaitlyn Chalker (WLU) def. Lisa Messier (SWA) 8-7 (7-2) |
